Enrichment of anammox bacteria at room temperature with activated sludge inocula
DOI:
https://doi.org/10.24850/j-tyca-2024-04-08Keywords:
Anammox, enrichment of anammox bacteria, reversible dissolved oxygen inhibition, nitrogen removalAbstract
The discovery of the anaerobic ammonium oxidation process (anammox) opens up new alternatives for nitrogen removal that are cheaper than traditional nitrification-denitrification systems. The enrichment of these bacteria is favored at warm temperatures (above 30 °C), similar to those achieved in cubans natural environments. In this work, the enrichment of anammox bacteria at room temperature was evaluated, with inocula that come from different activated sludge systems for the treatment of municipal wastewater in Cuba. The reactors are batch operated and are fed with synthetic medium with sufficient ammonium, nitrite and carbonates. Anammox activity begins to be detected, under controlled conditions, after 40 days. The average values obtained for the stoichiometric ratios, N-NO2-/N-NH4+ and N-NO3-/N-NH4+ were 1.16 and 0.22, respectively. The highest value of the maximum specific activity that is achieved in the enrichments is 0.160 gNT gSSV-1 d-1, operating with nitrogen removal rates between 150 and 350 mgNT l-1 d-1 and reaching an efficiency 93.8% nitrite removal media. The anammox activity in the stationary phase of growth is reversibly affected by the presence of OD, which is verified with assays in manometric systems.
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